ABSTRACT
Cycas revolute Thunb is a first class protected plant in China and has high medicinal value. As early as the Qing dynasty, it was recorded that Cycas was use to treat disease. Early research on Cycas mainly focused on classification, protection and breeding. In recent years, people began to study the main chemical constituents, chemical structure and pharmacological effects of Cycas, which laid a solid theoretical foundation for the further development and utilization of Cycas. At present, most of amentoflavone derivatives and hinokiflavone derivatives found in Cycas have the therapeutic effect on tumor. In addition to the antitumor activity, the extracts of Cycas also have antibacterial, antioxidant and immunological activities.
ABSTRACT
This paper aimed to introduce and review the antioxidant and material base of selaginella medicinal plants.On the basis of the systematic identification of selaginella medicinal plants,we found that the ethyl acetate extract and flavonoids could inhibit the activity of XOD,LOX or/and COX-2 to some extent.Fourteen biflavonoids,one flavonoid and five flavonoid glycosides have been isolated from three resource plants,among them five compounds were firstly reported and fifteen compounds were firstly isolated in selaginella plants.Amentoflavone (AME),robustaflavone and robustaflavone-4''-dimethylether are three common characteristic constituents of selaginella plants,which can act as marks of common active ingredients and be applied to evaluate the quality of selaginella.Further studies found that some plants had stronger anti-Coxsackie virus and anti-tumor effects,and their mechanisms were associated with inhibiting multiple oxidase and mitigating harm of free radicals due to whole function.
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This study was conducted to investigate the chemical constituents in the root of Dysosma versipellis (Hance) M. Cheng. The constituents were isolated by silica gel, lichroprep RP-C18 and pharmadex LH-20 column chromatography and the IR, MS, NMR, 2D-NMR spectroscopic analysis were employed for the structural elucidation. Ten compounds were isolated from the 95% ethanol extract of Dysosma versipellis, their structures were elucidated as dysoverine D (1), dysoverine F (2), dysoverine A (3), podoverine A (4), α-peltatin (5), rutin (6), kaempferol-3-O-β-D-glucopyranoside (7), quercetin-3-O-β-D-glucopyranoside (8), kaempferol (9) and quercetin (10). Compound 2 is a new compound, and compounds 1 and 3-6 were isolated from this plant fo r the first time.
ABSTRACT
The presentstudy is to investigate the chemical constituents of needles of Taxus canadensis. The constituent was isolated by various chromatographic methods, and the chemical structure was elucidated on the basis of spectral analysis. A new biflavone was isolated and identified as 3"'-hydroxy-4"', 7-dimethylamentoflavone.
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The present study investigated the chemical constituents of the roots of Stellera chamaejasme (Thymelaeaceae). One new biflavone glucoside (1), along with other thirteen known compounds (2-14), was isolated by repeated column chromatographic methods and their structures were elucidated on the basis of spectral analyses. The cytotoxic activities of selected compounds were evaluated against four human cancer cell lines (A549, BEL-7402, HCT-116, and MDA-MB-231) by the SRB assay method. Compound 9 showed remarkable cytotoxicity against BEL-7402 with IC50 value being 0.65 μg·mL(-1); compounds 7, 8, and 12 exhibited significant cytotoxic activity against A549 with IC50 values being 2.38, 1.57, and 2.35 μg·mL(-1), respectively.
Subject(s)
Humans , Antineoplastic Agents, Phytogenic , Chemistry , Pharmacology , Therapeutic Uses , Biflavonoids , Chemistry , Pharmacology , Cell Line, Tumor , Glucosides , Chemistry , Pharmacology , Inhibitory Concentration 50 , Molecular Structure , Phytotherapy , Plant Extracts , Chemistry , Pharmacology , Therapeutic Uses , Plant Roots , Chemistry , Thymelaeaceae , ChemistryABSTRACT
This study was aimed to establish an analysis method of amentotaxus biflavone distribution in rats' tissues, including the heart, liver, spleen, lungs, kidney, brain, stomach, large intestine and small intestine, in order to investigate its distribution characteristics in tissues after rats gavaged with amentotaxus biflavone. HPLC-UV was employed to determine contents of amentotaxus biflavone in rats' tissues. The intragastric administration dose of amentotaxus biflavone was 500 mg·kg-1. Rats were sacrificed by cervical dislocation 10 min, 0.5, 1, 2, 4, 8, 12 h after intragastric administration. Tissues of the heart, liver, spleen, lungs, kidney, stomach, large intestine, small intestine and brain were removed and dissected immediately. Distribution of amentotaxus biflavone in each tissue was determined after processing. The results showed that tissue in different range had a good linear range. The lowest detection limit was 0.125 ng. The RSD of intra and inter-day was less than 10%. The absolute recovery rate of amentotaxus biflavone in each tissue was between 75.07% and 89.80%. The relative recovery rate was between 92.00% and 107.00%. Amentotaxus biflavone in each tissue was stable in different tissues in refrigerator of -20° C for 15 days. It was concluded that there were relatively large concentration differences of amentotaxus biflavone in different tissues. After intragastric administration, amentotaxus biflavone was mainly distributed in the stomach, large intestine, small intestine, liver and kidney, and then the heart, lungs and spleen. It also distributed in brain tissues through the blood-brain barrier.
ABSTRACT
Objective: To study the chemical constituents from Selaginella doederleinii and their bioactivities. Methods: Column chromatography on silica gel, RP-C18, and Sephadex LH-20 columns was used to separate and purify the chemical constituents. The structures were elucidated by physicochemical properties and spectroscopic analyses. The inhibition of EtOAc fraction on the tumor cells was tested in vivo and in vitro. Results: Eleven compounds were obtained from EtOAc fraction and identified as flavonoids and phenolic acids. These compounds were asamentoflavone (1), robustafavone 4'-methyl ether (2), 5, 5″, 7, 7″, 4', 4‴-hexahydroxy-(2', 8″)-biflavone (3), 7, 4', 7″, 4‴-tetra-O-amentoflavone (4), 5, 5″, 7, 7″, 4', 4‴-hexahydroxy-(2', 6″)-biflavone (5), ginkgetin (6), isoginkgetin (7), quercetin-3-O-α-D-arabinfuranosi (8), nobiletin (9), gallic acid (10), and shikimic acid (11). Two alkaloids, berberine (12) and palmatine (13) were obtained from n-BuOH fraction. The bioassays indicated that EtOAc fraction could inhibit the growth of HeLa and HepG2 in vitro with IC50 values of 0.12 and 0.60 mg/mL, respectively. The tumor-inhibition rate in S180-bearing mice was 43%. Conclusion: Compound 5 is a new natural product, and compounds 3, 6-10, 12, and 13 are isolated from the plant for the first time.
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The biflavonoid 2",3"-dihydroochnaflavone, isolated from the Luxemburgia species (Ochanaceae), was treated with diazomethane to obtain the trimethyl-ether that was treated with pyridine/acetic anhydride to yield a new derivative, 5-acetil-7,4'-dimethyl-flavone-(3'→O-4'")-5"-acetil-7"-methyl-flavanone. The complete ¹H and 13C NMR data assignments of the new derivative were made by the one- and two-dimensional spectral analysis.
O biflavonóide 2",3"-diidroochnaflavona, isolada de espécies de Luxemburgia (Ochanaceae), foi tratada com diazometano e forneceu o éter trimetílico que, em seguida, foi tratado com anidrido acético/piridina obtendo-se um novo derivado, 5-acetil-7,4'-dimetil-flavona-(3'→O-4'")-5"-acetil-7"-metil-flavanona. Fez-se o completo assinalamento dos dados de RMN ¹H e 13C através da análise dos espectros uni- e bidimensionais do derivado.
ABSTRACT
Object To study the chemical constituents of the whole plant of Selaginella stauntoniana Spring. Methods Various chromatographic techniques were employed for the isolation and purification of its constituents, and structurally identified by spectral analysis (IR, UV, MS, 1HNMR, 13CNMR) and chemical evidence. Results Four compounds were identified from its extract as: emodin (Ⅰ), ginkgetin (Ⅱ), hinokiflavone (Ⅲ), amentoflavone (Ⅳ). Conclusion All the compounds were isolated in this plant for the first time; compound Ⅰ was found from the plants of Selaginellaceae Beauv. for the first time.